May 1, 2016

All positive ANA EIA screens will be automatically reflexed to ANA by IFA.
NOTE: Children under the age of 18 should not be screened with the
ANA-EIA assay. They should be screened by the ANA-IFA assay. Children that exhibit autoimmune symptoms tend to test negative on the EIA screen. Please order ANA by IFA [Test code 543] for patients less than 18 years of age.

Our Lupus Panel is now a Lupus Reflexive Panel [Test code 1663].

  • The initial screen will be: ANA by EIA, C3, C4, Rheumatoid Factor, and Thyroid Peroxidase Antibody.
  • If the ANA by EIA is positive, the first reflex will be to ANA by IFA.
  • If the ANA by IFA is positive, the second reflex will be the dsDNA Ab, IgG by IFA.
  • If the ANA by IFA has a pattern and titer of 1:80 or greater, the last reflex will be for the extractable nuclear antigens: Sm, Sm/RNP, SSA, SSB, SCL-70, and Ribosomal P.

If you want dsDNA Ab testing and you have not ordered the LUPUS Panel, please order test code 545.

If you have any question, please call Paul Hoyt, Laboratory Manager at 323-222-6688.